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KMID : 0368420080510020097
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Journal of Plant Biology
2008 Volume.51 No. 2 p.97 ~ p.97
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Analysis of flavonoids and characterization of theOsFNS gene involved in flavone biosynthesis in Rice
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Kim Jeong-Ho
Cheon Young-Min
Kim Bong-Gyu
Ahn Joong-Hoon
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Abstract
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The major flavonoids in rice leaves were analyzed via LC-MS/MS after their total flavonoid extracts were hydrolyzed. The most abundant flavones were apigenin, luteolin, and tricetin. Of these, tricetin was methylated at its 3′ and 5′-hydroxyl group to form tricin, which was probablyO-glycosylated. Both 3′-O-methylated luteolin and luteolin were found in theC-glycosylated form while apigenin wasC-glycosylated. We also cloned and characterizedOsFNS, which catalyzes the reaction from flavanone (naringenin) to flavone (apigenin). Analysis of the reaction product with recombinant OsFNS showed that it indeed converts naringenin to apigenin.
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KEYWORD
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flavones, flavone synthase, flavonoid analysis, Oryza sativa
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